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Soft Matter CryoTEM Workshop

TU/e, Laboratory of Materials and Interface Chemistry together with ThermoFischer Scientific (Former FEI Company) will organize the 6th International CryoTEM Workshop 2018, from April 16th - April 20th 2018

several examples of CryoTEM images and tomography

Advancements in cryo-transmission electron microscopy (cryoTEM) have made it possible to image objects from biological origin, such as viruses and proteins, now also reaching near atomic resolution. In recent years cryoTEM is also increasingly used to investigate soft matter of synthetic origin: assemblies of organic (macro)molecules and/or inorganic nanoparticles.

Since almost a decade, Eindhoven University of Technology and ThermoFischer Scientific (Formerly known as FEI Company) have worked together at the forefront of this new field trying to advance cryoTEM in materials science, both through developing dedicated microscopes but also through developing advanced tools and methods for sample preparation and imaging. This has allowed us to investigate a variety of soft and hybrid materials in their native hydrated state with nanometer resolution both in two and three dimensions. Using cryoTEM and cryo-electron tomography (3D cryoTEM) we have revealed the structure and morphology of assemblies of surfactants, lipids and (bio)polymers in solution, as well as at interfaces. But cryoTEM also allows us to study the interactions between organic and inorganic components, e.g. in the mineralization of collagen, generating artificial bone-like materials.

With this workshop we want to demonstrate the possibilities of cryoTEM, and to show you how to use this powerful technique, in your own research and in your own laboratory. The workshop is aimed at participants at PHD/post doc level and who are experienced in basic microscopy and who wish to extend their knowledge and practical experience in 2D and/or 3D cryo imaging. The workshop will consist of lectures from experts, but also of extensive practical hands-on sessions.

 

PROGRAM HIGHLIGHTS

  • TEM OF SOFT MATTER: WHY CRYO-TEM?
  • SAMPLE PREPARATION: VITRIFICATION
  • LOW DOSE IMAGING
  • INTERPRETATION OF IMAGES
  • 2D VS 3D: TOMOGRAPHY
  • DATA ACQUISTION
  • 3D RECONSTRCUTION AND VISUALIZATION

Soft Matter CryoTEM Workshop